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Ct value and interpretation


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3 replies to this topic

#1 thenovice

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Posted 06 January 2011 - 01:45 PM

Hi
I am comparing the gene expression levels between two groups (Treated and untreated).
How do I present the results? In terms of Delta Ct values or Ct values normalized to housekeeping gene?
Please advice! I Dont think I can use delta delta Ct since I have only 2 groups and I may not be able to plot a bar graph :(
I appreciate your suggestions
Veigas

#2 scolix

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Posted 08 January 2011 - 12:02 PM

use the control and substrate the treated values from control. assume control samples as 1 and any change is related to this control.

is this helpful?

#3 thenovice

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Posted 08 January 2011 - 06:11 PM

use the control and substrate the treated values from control. assume control samples as 1 and any change is related to this control.

is this helpful?

Hi Scolix
Thanks for the advice. I am going to use this now :) THanks for your time!
Veigas

#4 qPCRman

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Posted 12 January 2011 - 06:59 AM

Hi-

You need to normalize the Cts to the endogenous control- get the DCt and then compare the control to the target group for fold change. You can calculate the DDCt with two samples.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

and also the Livak paper.

Sam




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