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### #1 amarieu

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Posted 06 January 2011 - 08:44 AM

I need help ASAP.

I am in the middle of Recombineering and I have to make MacConkey agar plates.
The protocol calls for 40g agar in 1L with 1% galactose and 500ul chloramphenicol (final concentration of 12.5ug/ml)

I am making half: 20g in 500ml with 1% but i cannot figure out the chloramphenicol calculations. Our chl is at 50mg/ml. Thank you do much!

-FC

### #2 almost a doctor

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Posted 06 January 2011 - 09:52 AM

You need to apply  C1V1 = C2V2
Where C is concentration and V is volume, and 1 and 2 are the initial and final.

so in your case Chl is at C1=50mg/ml and you wonder V1.
C2=12.5ug/ml and V2=500ml.

All you need to do is put the number in the equation and V1= 0.125ml = 125ul

### #3 amarieu

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Posted 06 January 2011 - 09:58 AM

almost a doctor, on 06 January 2011 - 09:52 AM, said:

You need to apply  C1V1 = C2V2
Where C is concentration and V is volume, and 1 and 2 are the initial and final.

so in your case Chl is at C1=50mg/ml and you wonder V1.
C2=12.5ug/ml and V2=500ml.

All you need to do is put the number in the equation and V1= 0.125ml = 125ul