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culture media for R1 cells


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#1 metta

metta

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Posted 05 January 2011 - 03:21 AM

can some one suggest me a good culture condition for R1 ES cells.

currently i am using (for 50 ml of media)
KO DMEM (invitrogen) - 41.0 ml
FBS (A&E scientific) - 7.5 ml
pen/strep/glu (invitrogen) - 0.5 ml
NEAA (lonza) - 0.5 ml
2-mercapto ethanol(sigma 0.1 mM) - 0.5 ml
LIF (ESRGO) (1:20 dil) - 25 micro liters

the cells are growing normally but i donot have good success with electroporations. i am thinking of shifting to invitrogen's serum replacement.

thanks

regards
m




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