Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cDNA synthesis


  • Please log in to reply
3 replies to this topic

#1 thenovice

thenovice

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 04 January 2011 - 10:30 AM

Hi
I have a Invitrogen's "SuperScript® III First-Strand Synthesis System" kit used only once but is 2 years old. Is it wise to use it for cDNA synthesis now? The kit literature says "Guaranteed stable for 6 months when properly stored". It hasnt gone through any freeze thaw cycles. Just wondering if anyone has used an expired kit and still got results??? Its an expensive kit. So just want to make sure before buying a new one!
Thanks

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,668 posts
565
Excellent

Posted 04 January 2011 - 01:07 PM

The general rule is "when in doubt, throw it out" - even if it does work, how do you know that it is transcribing the cDNA effieciently which is very important if you are planning on doing any real-time PCR (q-PCR).

#3 thenovice

thenovice

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 04 January 2011 - 01:34 PM

The general rule is "when in doubt, throw it out" - even if it does work, how do you know that it is transcribing the cDNA effieciently which is very important if you are planning on doing any real-time PCR (q-PCR).


Thanks Bob :)

#4 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 237 posts
11
Good

Posted 04 January 2011 - 11:04 PM

If it was properly stored at -20 I'd check if it still works and I'd use it.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.