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Tris Glycine Buffer


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4 replies to this topic

#1 akhairullah

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Posted 04 January 2011 - 08:07 AM

Hi everyone

I'm new to this forum, would appreciate some help!

I came across Tris Glycine Buffer in one of my lab's protocols for immunostaining.

Google told me that it is used in many other techniques such as westerns and sds-page but cant seem to find out the purpose it serves in immunostaining

Can anyone shed some light?

Thanks

#2 mdfenko

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Posted 04 January 2011 - 08:41 AM

western is immunostaining...

tris-glycine buffer is the transfer buffer.

talent does what it can
genius does what it must
i used to do what i got paid to do


#3 BioMiha

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Posted 04 January 2011 - 11:01 PM

Tris glycine is the running buffer!

#4 akhairullah

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Posted 05 January 2011 - 05:50 AM

Thanks for the replies

But I think I might have been vague or incorrect when I said 'immunostaining'

What we are doing is using tissue sections of rat brains and staining with antibodies conjugated with fluorescence

There is one step midway through the protocol that requires me to incubate the sections with Tris Glycine Buffer for 15 minutes

The purpose of this step is what eludes me

#5 BioMiha

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Posted 05 January 2011 - 10:56 AM

I'd say you can skip the tris glycine unless the pH is important.




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