Tris Glycine Buffer
Posted 04 January 2011 - 08:07 AM
I'm new to this forum, would appreciate some help!
I came across Tris Glycine Buffer in one of my lab's protocols for immunostaining.
Google told me that it is used in many other techniques such as westerns and sds-page but cant seem to find out the purpose it serves in immunostaining
Can anyone shed some light?
Posted 04 January 2011 - 08:41 AM
tris-glycine buffer is the transfer buffer.
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 05 January 2011 - 05:50 AM
But I think I might have been vague or incorrect when I said 'immunostaining'
What we are doing is using tissue sections of rat brains and staining with antibodies conjugated with fluorescence
There is one step midway through the protocol that requires me to incubate the sections with Tris Glycine Buffer for 15 minutes
The purpose of this step is what eludes me
Posted 05 January 2011 - 10:56 AM