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establishment of primary cell line from biopsy


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#1 Nya

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Posted 04 January 2011 - 07:11 AM

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance :)
Happy 2011 everyone

Nya

#2 Irene G

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Posted 11 January 2011 - 02:47 AM

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance :)
Happy 2011 everyone

Nya


Try disgregation with collagenase, maybe it could help!

#3 Nya

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Posted 11 January 2011 - 03:57 AM

Thanks so much for your suggestion! :)




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