2 replies to this topic

[Nya]

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance
Happy 2011 everyone

Nya

[Irene G]

Nya, on 04 January 2011 - 07:11 AM, said:

Hi everyone,

I'm trying to establish primary cell lines from bladder cancer samples. I keep trying, but after a while I still cant have any growth at all!

I've been using this protocol where I cut the sample into little pieces, seed it and then add some F-12 medium + non essencial a.a., dextrose, insulin, glutamin, P+S as antibiotics and FBS.

Cant really understand where is the problem, no article describes this problem or how to fix it, im really getting worried.

Thank you in advance
Happy 2011 everyone

Nya

Try disgregation with collagenase, maybe it could help!

[Nya]

Thanks so much for your suggestion!