Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

labeling a protein


  • Please log in to reply
3 replies to this topic

#1 Mssmilie

Mssmilie

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 January 2011 - 11:52 PM

I have a commercially available protein (Rec A). Is it possible to fluorescent label the protein. I have seen that in most cases the label is added in the purification step. I am new to this field and need serious help? Thanks

#2 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 235 posts
9
Neutral

Posted 04 January 2011 - 06:08 AM

It is generally possible to add a label to any protein even after purification. There are a number of vendors that sell labeling kits (Invitrogen and Pierce come to mind, but no doubt there are others as well) for flourescently labeling your protein. The only thing that could seriously hinder labeling is if your protein were in a Tris buffer, because the majority of labeling reactions go via the amino groups. Because Tris is an amine it acts as a decoy for the labeling and hence lowers the yield of successfully labeled protein dramatically. If this is the case you can dialyze your protein and you should be OK.
Best wishes.
Miha

#3 antibodymania

antibodymania

    member

  • Active Members
  • Pip
  • 27 posts
17
Good

Posted 04 January 2011 - 11:12 AM

Azide also contains amine groups that can effect coupling. If you purified the protein yourself, then this may not be a concern. But some commercially available products add azide as a preservative.

#4 Mssmilie

Mssmilie

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 04 January 2011 - 05:38 PM

Thank you very much for the help. I will look into these labels from these vendors.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.