Posted 03 January 2011 - 05:59 PM
Posted 04 January 2011 - 02:47 AM
The DNA-polymerase can only sinthesize in 5' to 3' direction. Your fwd primer is in on the beginning of the + strand in 5' to 3' direction, and your reverse primer is on the beginnig of the -strand in 5' to 3' (or if it easier to visualize for you: on end of the - strand in 3' to 5' direction). So after you amplify your desired gene with this primers you will get the dsDNA with fwd and reverse primer (http://lslab.lscore....dria/primer.htm).
Why when we make Forward primer we keep the sequence as it is without doing it in complementary, while we do reverse complement in the reverse primers. any clue
Also, there are lots of other useful sites about primer design, just google it. Here are some I came across: PCR_Primer Design, primer_design
Edited by lab_microbe, 04 January 2011 - 04:53 AM.