Posted 03 January 2011 - 10:29 AM
I am isolating rna from a legume and following the GITC method. What i am unable to understand is that the pellet I get after isopropanol addition is yellow and stays yellow after 70% ethanol wash. I run the rna in formaldehyde gel and the quality of rna is good. Is the rna sample containing some contaminants ? Will it interfere in RT-PCR?
Posted 05 January 2011 - 04:59 AM
Posted 05 January 2011 - 12:47 PM