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rna isolation


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#1 deespike

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Posted 03 January 2011 - 10:29 AM

Hi all
I am isolating rna from a legume and following the GITC method. What i am unable to understand is that the pellet I get after isopropanol addition is yellow and stays yellow after 70% ethanol wash. I run the rna in formaldehyde gel and the quality of rna is good. Is the rna sample containing some contaminants ? Will it interfere in RT-PCR?

Plz help

#2 ElHo

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Posted 05 January 2011 - 04:59 AM

This once happened to me when using old isopropanol for precipitation. Although I didn`t observe any problems concerning RNA quality, I would repeat RNA isolation if possible using fresh isoprop.

#3 bob1

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Posted 05 January 2011 - 12:47 PM

I would suspect phenolic contamination (from the legume) of the RNA, it may or may not interfere with down-stream applications.




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