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Preparing M&S medium


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4 replies to this topic

#1 lyok

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Posted 02 January 2011 - 10:26 AM

Hallo all,

I had to make a M&S medium (Murashige and Skoog). And I had to make 3 "bottles" of medium and then add those together. But why did I need to make those "in between solutions" (stocksolutions?)


I think it has something to do with preventing precipitation? But what components would precipitate then?

#2 johnmartin

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Posted 14 March 2011 - 01:20 AM

I also have no idea that what components are needed to participate in Murashige and Skoog. if you get any idea about it, please also let me know.
irrigation systems design

#3 strawberry

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Posted 29 April 2011 - 12:42 PM

didn't get your question :unsure: ..but i think it has something to do with contamination..

i mean it could be better doing each separately to minimize contamination..

in other terms..you'll not be happy if all your media got contaminated ;)

#4 josse

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Posted 30 April 2011 - 01:10 AM

Hallo all, sorry for the late reply. The ingrediets I need to use are:

solution1:
MgSO4•7H2O
KNO3
NH4NO3

Solutionb2:
KH2PO4

Solution3:
CaCl2•2H2O


And it was the solutions 1, 2 and 3 I had to prepare in diffrent bottles to prevent precipitation.
(after this I simply had to put them toghether with the rest , solutions 4, 5 and vitamines were just seperated because those where allready in stock)
SO I wonder what is in solutions 1,2 and/or 3 that you cant mix?


solution 4:
H3BO3
MnSO4•H2O
ZnSO4•H2O
KI
NaMoO4•2 H2O
CuSO4•5H2O
CoCl2•6H2O

solution5:
FeNaADTA was used in stead of FeSO4•7H2O

and vitamines
myo-inositol
nicotinic acid
thiamine•HCl
pyridoxine•HCl
glycine






Its not to minimize contamination, because you make the changes on contamination bigger by doing it like this.. (since you autoclave more then 1 bottle and you add them toghether afterwards..)

So any ideas?

Edited by josse, 30 April 2011 - 01:14 AM.


#5 mdfenko

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Posted 02 May 2011 - 06:55 AM

i don't know why 1 can't be mixed with 2 or 3 but 2 and 3 can't be mixed because capo4 will form and precipitate.
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