Which acetat for a DNA isolation
Posted 02 January 2011 - 08:08 AM
What is the difference in using whether potassium or sodium acetate for a DNA preparation when I am
doing the alcohol precipitation?
Posted 02 January 2011 - 02:22 PM
Posted 04 January 2011 - 03:41 AM
Did I understand correctly?
-> If I am doing a plasmid isolation the sodium acetate is used to neutralize the SDS lysis buffer
-> If I am doing a isolation of genomic DNA I use potassium acetate to precipitate potassium dodecyl sulfate (or did you mean sodium dodecyl sulfate?)
Posted 04 January 2011 - 04:55 AM