How sensitive are QPCR machines compared to fluorescent plate readers using Sybr I and looking for dsDNA? i.e. how many ng of Sybr I stained dsDNA is A ) detectable and B ) differentiable/with what resolution? We have an old plate reader that can detect 1ng of Sybr I stainted Lambda in 100ul solution, with a resolution of about 1-2ng.
I see that many QPCRs claim to be able to resolve 5000 from 10000 copies of a ~350 nucleotide RNA, which would amount to attograms of material?
Would it also be possible to make this (semi) quantitative by using know amounts of dsDNA as a standard?
Edited by grkn, 30 December 2010 - 03:00 PM.