Are protease inhibitors overrated ?
Posted 30 December 2010 - 11:50 AM
Posted 30 December 2010 - 12:08 PM
some proteases work very quickly and will maintain some activity at low temperatures. some, what you may consider, minor handling differences may lead to large differences in yield and condition of your protein of interest.
the best commercial inhibitor cocktails cover all known classes of protease.
if you want to make your own cocktail then you will have to do the same. keep in mind that some inhibitors require careful handling and some are relatively labile.
genius does what it must
i do what i get paid to do
Posted 30 December 2010 - 01:27 PM
Keep in mind that if your protein is easily degraded, the price of a protease inhibitor in quickly paid back by a better yield of the protein. Hope you find a good protocol!