hi. i am doing gst-pulldown however i got inconsistent results sometimes. I just want to ask what major factors could have caosed this inconsistent results....
i would like to measure the activity of a certain protein in the cell and i expect that the amount of the protein in the pulldown will be different ( increase or decrease) however, when i did the pulldown again, all signals are the same. I used exactly the same conditions.
what could have be possible reasons for this. thank you....
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 3,265 posts
an elder emeritus
198
Excellent
Excellent
Posted 30 December 2010 - 08:56 AM
are you sure you had excess antibody?
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
