hi. i am doing gst-pulldown however i got inconsistent results sometimes. I just want to ask what major factors could have caosed this inconsistent results....
i would like to measure the activity of a certain protein in the cell and i expect that the amount of the protein in the pulldown will be different ( increase or decrease) however, when i did the pulldown again, all signals are the same. I used exactly the same conditions.
what could have be possible reasons for this. thank you....
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mdfenko
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Posted 30 December 2010 - 08:56 AM
are you sure you had excess antibody?
talent does what it can
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