Posted 30 December 2010 - 12:27 AM
i would like to measure the activity of a certain protein in the cell and i expect that the amount of the protein in the pulldown will be different ( increase or decrease) however, when i did the pulldown again, all signals are the same. I used exactly the same conditions.
what could have be possible reasons for this. thank you....
Posted 30 December 2010 - 08:56 AM
talent does what it can
genius does what it must
i used to do what i got paid to do