Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

gst- pulldown

  • Please log in to reply
1 reply to this topic

#1 soymilk14



  • Active Members
  • PipPipPipPipPip
  • 81 posts

Posted 30 December 2010 - 12:27 AM

hi. i am doing gst-pulldown however i got inconsistent results sometimes. I just want to ask what major factors could have caosed this inconsistent results....

i would like to measure the activity of a certain protein in the cell and i expect that the amount of the protein in the pulldown will be different ( increase or decrease) however, when i did the pulldown again, all signals are the same. I used exactly the same conditions.

what could have be possible reasons for this. thank you....

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,369 posts

Posted 30 December 2010 - 08:56 AM

are you sure you had excess antibody?

talent does what it can
genius does what it must
i used to do what i got paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.