Can anyone tell me whether this is possible and the wehat % of agarose/polyacryamide should i try?
This step is utmost important because if i fail in doing so, i may have to do single digestion for plasmid and gene and pick colonies forever (dont mention 5-phosphatase and terminal transferase, we dont have it in lab).So, plz help~
by the way, i need to isolate the 803bp DNA framgent from the gel in the later steps. So, i need a lot amount of fragments.
Edited by sexymonkey, 29 December 2010 - 10:23 PM.