Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cotransfection


  • Please log in to reply
1 reply to this topic

#1 martapaterlini

martapaterlini

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 20 February 2002 - 02:45 PM

Hi,
I am planning to do a stable cotransfection in PC12 cells.
How could I set a double selection? Is it better to start with one transfection, do the selection and then carry on with te second transfection/selection or what?
Thank you,
marta

#2 labslave

labslave

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 21 November 2002 - 10:10 AM

It is better to create a stable cell line with one of your plasmids. Then do a single transfection. A cotransfection would cause you to add too many antibiotics and may harm the cell.

Hope that works!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.