Refrigerator left open.....is everything ruined?? :(
Posted 29 December 2010 - 01:27 PM
Im currently testing the antibodies but Im really concerned about the proteins and enzymes we use!! Do you think the proteins and antibodies which were on the shelf of the door have been completely ruined?? What about the ones which were still on ice?? We use some proteins like fibronectin to plate cells....do u think it is ruined if it was on the shelf of the door?? Ill try and plate some cells on it to see if it works....i really dnt kno how to test proteins which other ppl use!
Urrgghhh.....my labmates and PI are all lovely ppl but I wudnt blame them if they kick me out of the lab for this one!! I dont even kno what to say to them wen they come bak!!
Posted 29 December 2010 - 02:05 PM
This happened to me too, not 12-15 hours but 3 weeks(!!), due to "mis-communication" between me, my labmates, and PI. On top of that, I made another big mistake (killed an S200 column). After apologizing to my PI and everyone in the lab (I wasn't kicked out), I guess how they understand it is that this kind of mistake is just something that happens (shit happens). What my PI said (and other veteran in this forum said) is make sure that you won't do it again.
Also before I leave lab, I always double check everything (make sure fridge/freezer doors are closed, no bunsen burner on, no lamp on, oven is off, microwave looks normal, etc)
If "room temp" is 4-8C (since this is winter) then I hope it's still ok since it's close to ice and all..
Posted 29 December 2010 - 02:40 PM
I kinda chek whether ive put everythin bak in the freezer....but i never figured tht i cud do tht since the door closes on its own!! ....thnks for the suggestion tho....ive made a cheklist of things wich ill chek everyday before i leave!
It is cold outside but the lab is at about 20C.....
Posted 30 December 2010 - 08:53 AM
genius does what it must
i do what i get paid to do
Posted 06 January 2011 - 01:13 PM
Posted 06 January 2011 - 09:29 PM