Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

query regarding analysis- relative gene expression

  • Please log in to reply
No replies to this topic

#1 kalpanak



  • Members
  • Pip
  • 2 posts

Posted 29 December 2010 - 03:52 AM

Hi all,

I have a query regarding analysis of real time PCR results. I am comparing gene expression levels of two gene isoforms with gapdh as internal reference. i have to compare relative gene expression levels in drug treated and untreated samples. Since delta Ct (Ct of target-Ct of reference) is inversely proportional to mRNA levels, i inverse delta Ct and compare between two isoforms in the samples. but, I have not come across any paper that use 1/delta Ct to compare expression levels. Is it correct to compare that way? In my case, fold change by delta delta Ct do not provide adequate explanations.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.