Amplification of region from sperm RNA
Posted 28 December 2010 - 08:10 AM
Posted 29 December 2010 - 04:03 AM
Posted 31 December 2010 - 12:06 AM
And cDNA is better for storage than RNA.
Posted 31 December 2010 - 12:08 AM
Posted 03 January 2011 - 09:08 PM
Another thing, if you know the sequence of the region you want to amplify, design specific reverse primer, instead of polyT.
Thanks . I think storing at -70 helping me out, and even avoiding repeat freeze thaws. Thanks