Estimation of DNA Quantity on an Agarose Gel
Posted 27 December 2010 - 10:34 PM
Posted 29 December 2010 - 08:20 AM
Posted 29 December 2010 - 10:39 PM
just use an marker with bands of different size and intensities
So with which of the bands of the marker would you recommend that I compare? The one that is of similar size to my band, or the one that is of similar intensity?
Posted 30 December 2010 - 09:58 AM
Posted 30 December 2010 - 12:11 PM
State-of-the-art an much more exact is qPCR, as the here the exponential and therefore quantifiable part of the reaction is used. But it's expensive and you need a real-time machine.
You might try a competitive PCR as alternative.
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.