Suitable vector backbone for in vitro translation in Dros.embryos

Started by srm87, Dec 27 2010 07:20 PM

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#1 srm87

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Posted 27 December 2010 - 07:20 PM

I need to assemble a series of constructs that will allow me to test the requirement of 5'/3'UTR regions of target mRNAs for translational control.
Does anyone have a good vector backbone suggestion i could use? preferably involving a T7 promoter, luciferase coding regions with multiple cloning sites to insert 5'/3'UTR regions and a poly A tail
Thanks!