Hello,
I am isolating DNA from several mouse organs using DNAzol. 1:10 TE to dissolve the end pellet has given decent DNA yields for the size and type of my samples, but I recently saw a DNAzol protocol online from another company stating that "DNA isolated from DNAzol does not solubilize well in TE"- and that NaOH should be used instead.
Although the DNA yields seem fine, we are looking at samples to "rule out" a sequence- and I want to make sure that the DNA is well (and not partially) dissolved. Does anyone have any experience, knowledge or advice with this?
Thank you!
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