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143 kDa protein


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3 replies to this topic

#1 samita

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Posted 26 December 2010 - 03:46 PM

What percentage of PAGE will be good to resolve 143 kDa protien. 6 % I read but I am not sure about it. I try 10 percent but it does not resolve properly and stays at the top of the gel.

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#2 Inmost sun

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Posted 27 December 2010 - 02:12 AM

run a gradient SDS gel ranging from 2-4% to 16-20%

#3 samita

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Posted 27 December 2010 - 08:09 AM

I never heared about the gradient SDS, I am making just a staking gel with 5 %.....what is the procedure of making a gradient SDS gel.???

run a gradient SDS gel ranging from 2-4% to 16-20%



#4 mdfenko

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Posted 30 December 2010 - 08:44 AM

what are the other protein sizes from which you have to resolve the 143 kDa protein? if there is enough difference then you can use a 7-7.5% gel for good resolution.

if you don't know how to pour a gradient then you probably don't have the equipment necessary. however, you can buy gradient gels if you need them.
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