Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

IP after sucrose density gradient fractionation

  • Please log in to reply
No replies to this topic

#1 srm87



  • Members
  • Pip
  • 2 posts

Posted 26 December 2010 - 07:07 AM

I'm having some issues thinking about an appropriate time line to isolate interactors of my protein of interest within polysome fractions after density gradient fractionation.
I'd like to add in a DSP crosslinker to stabilize any transient/weak interactors.
Would it make more sense to add the cross linker before fractionation and IP, or fractionate and then crosslink-IP?
Any suggestions/ alternative ideas are welcome!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.