I'm having some issues thinking about an appropriate time line to isolate interactors of my protein of interest within polysome fractions after density gradient fractionation.
I'd like to add in a DSP crosslinker to stabilize any transient/weak interactors.
Would it make more sense to add the cross linker before fractionation and IP, or fractionate and then crosslink-IP?
Any suggestions/ alternative ideas are welcome!
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IP after sucrose density gradient fractionation
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