For example, I often come across the term 'forward and reverse primers', where one sequnce is complementary to the other sequance, such as:
Furthermore, I also come across websites which provide sequences for specific loci (STR's), but these sequnces are not complementary, for example:
CAA ACC CGA CTA CCA GCA AC
GAG CCA TGT TCA TGC CAC TG
What is the difference and which would you use as PCR primers if you were targeting specific loci?
Edited by Baileys, 23 December 2010 - 09:26 AM.