Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

centrifuge of cells


  • Please log in to reply
3 replies to this topic

#1 andy

andy

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 23 February 2002 - 10:23 AM

How long and how fast should a solution be centrifuged to obtain a pellet of epithilial cells.

#2 Sean Peel

Sean Peel

    member

  • Active Members
  • Pip
  • 17 posts
0
Neutral

Posted 26 February 2002 - 01:23 PM

The standard method I use (for connective tissue cells, primary and transformed) is 5 to 10 minutes at 180g.

I would expect this to work for epithelial cells too

#3 flashboy

flashboy

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 47 posts
1
Neutral

Posted 12 March 2002 - 03:20 AM

i use 400g for 5 mins

#4 Tim

Tim

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 24 July 2002 - 01:38 AM

Dear Andy
400g for 7 minutes is the most optimum, I find




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.