3 replies to this topic

[andy]

How long and how fast should a solution be centrifuged to obtain a pellet of epithilial cells.

[Sean Peel]

The standard method I use (for connective tissue cells, primary and transformed) is 5 to 10 minutes at 180g.

I would expect this to work for epithelial cells too

[flashboy]

i use 400g for 5 mins

[Tim]

Dear Andy
400g for 7 minutes is the most optimum, I find