Hi,
That's say if I found an inhibitor to a pathway. Among many points in the pathway, how can I find out that which point the inhibitor targets. Can I perform a gel shift assay?
0
3 replies to this topic
#2
bob1
-
- Global Moderators
-
- 4,340 posts
Thelymitra pulchella
222
Excellent
Excellent
Posted 22 December 2010 - 12:58 PM
What sort of inhibition? DNA? RNA? protein? each has their own method of detecting interaction.
#3
yi198720022004
-
- Active Members
-
- 14 posts
member
0
Neutral
Neutral
Posted 22 December 2010 - 08:11 PM
bob1, on 22 December 2010 - 12:58 PM, said:
What sort of inhibition? DNA? RNA? protein? each has their own method of detecting interaction.
It's like a signaling transduction pathway.
Edited by yi198720022004, 22 December 2010 - 08:11 PM.
#4
dpo
-
- Active Members
-
- 67 posts
Enthusiast
1
Neutral
Neutral
Posted 23 December 2010 - 12:00 AM
You can try to rescue the effect you get from the inhibitor. For instance, suppose you have an inhibitor acting on the Wnt signaling pathway. If overexpressed beta-catenin rescues the phenotype of the inhibitor, you know the inhibitor is acting upstream of the activation of b-ctn, for instance at the binding of the Wnt ligand to the receptor. A lot of pathways can be activated by different exogenous stimuli, so you'll have to read about your pathway and find some steps where you can intervene ...
