Do you get insoluble proteins also in whole cell lysate?
Posted 21 December 2010 - 11:48 PM
I have this general question. When you lyse the whole cell pellet with any lysis buffer containing detergents like NP40 etc., do you get insoluble proteins also??? Because in general protocols we add lysis buffer in the pellet, keep it at ice for some time and then spin to collect the supernatant. Eventually we throw the pellet.What if you are looking for some specific protein and that goes in the pellet ...I may be wrong coz we are adding detergents to disrupt the membrane. But just wanted to check with people here on the forum. Hope somebody would be able to understand
Posted 22 December 2010 - 08:54 AM
genius does what it must
i do what i get paid to do
Posted 30 December 2010 - 02:04 PM
Posted 31 December 2010 - 05:45 AM