My friend bought an antibody(solid) from R&D systems .But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1.Purified recombinant human TGFβ1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin
,the reference from R&D says.
Here's the problem,how to reconstitute an antibody?Should she use a PBS or simply sterile water to reconstitute?Does anybody have a specific protocol?Thanks in advance!
If you need more information,you can check it by clicking the link belowhttp://www.rndsystem...m/pdf/240-b.pdf
Edited by damon, 21 December 2010 - 10:59 PM.