6 replies to this topic

[damon]

My friend bought an antibody(solid) from R&D systems .But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1.Purified recombinant human TGFβ1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin,the reference from R&D says.
Here's the problem,how to reconstitute an antibody?Should she use a PBS or simply sterile water to reconstitute?Does anybody have a specific protocol?Thanks in advance!
If you need more information,you can check it by clicking the link below
http://www.rndsystem...m/pdf/240-b.pdf

Edited by damon, 21 December 2010 - 10:59 PM.

[protolder]

damon, on 21 December 2010 - 10:58 PM, said:

My friend bought an antibody(solid) from R&D systems .But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1.Purified recombinant human TGFβ1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin,the reference from R&D says.
Here's the problem,how to reconstitute an antibody?Should she use a PBS or simply sterile water to reconstitute?Does anybody have a specific protocol?Thanks in advance!
If you need more information,you can check it by clicking the link below
http://www.rndsystem...m/pdf/240-b.pdf

Hola, the supplier says the correct way to reconstitute, first prepare the 4mM HCL. Take 0,5-1ml and dissolve 0.50r 1mg of BSA. filter in sterility the solution and add to the liophilized powder the amount of solution necessary to have 20ug/ml.     0.5 ml to 10ug of powder or 2.5ml for 50 ug. the amount of powder will be in the vial isn´t it?. Buena suerte y Feliz Navidad

[damon]

Thanks for helping me.Some people may fed up with this stupid question.However,as far as I am concerned, success depends on details.One more question, does liophilized here mean lyophilized?

protolder, on 21 December 2010 - 11:27 PM, said:

damon, on 21 December 2010 - 10:58 PM, said:

My friend bought an antibody(solid) from R&D systems .But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1.Purified recombinant human TGFβ1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin,the reference from R&D says.
Here's the problem,how to reconstitute an antibody?Should she use a PBS or simply sterile water to reconstitute?Does anybody have a specific protocol?Thanks in advance!
If you need more information,you can check it by clicking the link below
http://www.rndsystem...m/pdf/240-b.pdf

Hola, the supplier says the correct way to reconstitute, first prepare the 4mM HCL. Take 0,5-1ml and dissolve 0.50r 1mg of BSA. filter in sterility the solution and add to the liophilized powder the amount of solution necessary to have 20ug/ml.     0.5 ml to 10ug of powder or 2.5ml for 50 ug. the amount of powder will be in the vial isn´t it?. Buena suerte y Feliz Navidad

[protolder]

yes sorry

[damon]

Everyone makes a mistake.It's not a big deal.The point is that you just want to help people ASAP and it's very important.   

protolder, on 22 December 2010 - 12:06 AM, said:

yes sorry

[BioMiha]

I am totally lost here. I really don't understand, what you mean by "But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1." Is your friend trying to reconstitute the Ab or the TGFβ1? Abs are usually reconstituted in dH20 to a concentration that is recommended by the manufacturer so as to get a salt concentration that is optimal for the Ab.

[damon]

Sorry,it's the TGF beta 1.

BioMiha, on 22 December 2010 - 05:33 AM, said:

I am totally lost here. I really don't understand, what you mean by "But she was confused when she tried to RECONSTITUTE it,which is called TGF beta 1." Is your friend trying to reconstitute the Ab or the TGFβ1? Abs are usually reconstituted in dH20 to a concentration that is recommended by the manufacturer so as to get a salt concentration that is optimal for the Ab.