Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Sf9 contamination


  • Please log in to reply
2 replies to this topic

#1 BSturt

BSturt

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 21 December 2010 - 09:53 AM

I am growing Sf9 insect cells for baculovirus expression system. My only problem is fungus infection that seems to occur randomly. Any suggestions? Antibiotics or antimycotics don't agree with insect cells are typically not added to media.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,728 posts
399
Excellent

Posted 21 December 2010 - 01:50 PM

The only solution is to clean your equipment/room, change your solutions and maintain good sterile technique.

#3 protolder

protolder

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts
9
Neutral

Posted 21 December 2010 - 10:14 PM

Hola, I donīt know why do you say it about antibiotics and antifungus.Iīm working with insect cells since 80īs with fungizone P/S first, and after I changed to fungizone, amphotericin, and I havenīt problems with transfections, infections, titering and expressions and sometimes I have some contamination too. If you donīt want use them try to cure your infection and eliminate the antibiotic, antimicotic after. Buena suerte y Feliz Navidad




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.