Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

delta-delta-Ct method


  • Please log in to reply
1 reply to this topic

#1 moljul

moljul

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 130 posts
2
Neutral

Posted 21 December 2010 - 08:08 AM

how could i apply the delta-delta-Ct method to my real time PCR results, when target gene (gene of interest) is not expressed ("undetermined") in my healthy control?
is it allowed to insert Ct 45, to complete the formula??

#2 qPCRman

qPCRman

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 12 January 2011 - 07:09 AM

Hey

You can replace it with Ct=40 (max number of cycles). I use RealTime StatMiner software and it does it automatically. You can set a filter to see how many times this appeared Undetemined amongst your biological replicates. Undetermined may also suggest you don't have anything in your control, so that may be significant. You just have to setup your filters.

Take a look at this PCR whitepaper http://www.integromi...qPCR_Statistics

and also the Livak paper.

Sam




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.