pGEX vectors and GST expression
Posted 21 December 2010 - 07:51 AM
I'm using a antibody purification protocol that calls for me to incubate serum with antigen (GST fusion) and GST protein.
Thanks for your help.
Posted 21 December 2010 - 11:09 PM
Posted 22 December 2010 - 02:30 AM
Edited by MyProteinBulliedMe, 22 December 2010 - 02:30 AM.
Posted 29 December 2010 - 07:21 AM
Regarding the other comentary, sometimes this kind of plasmids have a protease target between GST gene and the MCS. For example, I have noticed in the case of factor Xa, i am convinced that there is a specific degradation of the recombinant protein when the complete protein is been producing due to traces of bovine factor Xa (maybe ppm) which are contaminating the media and the lysed extract also. Due to this reason we can have GST alone and GST plus the target protein.