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primary astrocyte culture


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#1 ggrrrmmmlllbbb

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Posted 21 December 2010 - 07:03 AM

hello,

I am a young scientist and for about a year now I have been trying to optimize my protocol to establish primary newborm rat astrocyte cell cultures.
The procedure an sich is not a problem, I am just having lots of problems due to infections.

I used have a lot of fungal infections but this problem seemed to be solved by adding some amphotericin B in my culture medium.
Today I discovered a yeast infection. This is the first time I encouter this. Does anyone know what it is coming from, how to avoid it or even how te clean my cultures from it?

many thanks

ps: is it true that it might be due to the fact that I just started to bake my own bread? I keep on reading this on some fora

Edited by ggrrrmmmlllbbb, 21 December 2010 - 07:04 AM.


#2 scolix

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Posted 29 December 2010 - 11:37 AM

possible. or even of you drink beer and work with cells, yeast infection is possible.

try to work aseptic as possible.




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