Posted 20 December 2010 - 11:41 AM
Posted 11 January 2011 - 02:52 AM
Posted 11 January 2011 - 03:09 AM
99% of the time fungal contamination will be coming from the CO2 Incubator. Flasks and dishes need to be wiped with 70% IMS before being put back in the incubator. Direct heat incubators have no fan units and are easier to clean and maintain. Regular water changing i.e. every week will also reduce the chance of fungal contamination.
Try and grow all cells in flasks with a filtered top (0.22uM) which will act as a barrier for all forms of contamination.
Hope this is useful.