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Transfer plasmid toAgrobacterium LBA4404

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#1 anonymous



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Posted 04 April 2001 - 09:00 PM

I want to tranfer a plasmid 12kd to DH5a(E.coli) and then get enough plasmid DNA to transform the agrobacterium LBA4404 ,but I did that for five times ,I only got one clone in two of which,and I got DNA from the clone then tranformed the LBA4404 in the way of freeze-thaw, but got nothing.Who can tell me why and how to solve the difficuty,thanks! naizhong

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