I recently tried to culture TMD8 cells (a diffuse large B cell lymphoma cell line) in the lab. I used standard culture condition: RPMI + 10% FCS (or IMDM + 10% human serum) + P/S + 50uM beta-ME. The cells were got from another lab in medium. They grew for a short period of time and then began to die quickly. I tried many ways to rescue the cells. I recovered live cells from the mass of dead cells by spin down extremely slow (~200 to 300 rpm) and culture them in small volume (by which way I have successfully rescured another DLBCL cell line); or increase the serum concentration to 20%, but I just cannot stop the cell dying. Are there any one ever working with this cell line and can give me some suggestions? Thank you!
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