Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

TMD8


  • Please log in to reply
No replies to this topic

#1 Iris74

Iris74

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 20 December 2010 - 08:47 AM

I recently tried to culture TMD8 cells (a diffuse large B cell lymphoma cell line) in the lab. I used standard culture condition: RPMI + 10% FCS (or IMDM + 10% human serum) + P/S + 50uM beta-ME. The cells were got from another lab in medium. They grew for a short period of time and then began to die quickly. I tried many ways to rescue the cells. I recovered live cells from the mass of dead cells by spin down extremely slow (~200 to 300 rpm) and culture them in small volume (by which way I have successfully rescured another DLBCL cell line); or increase the serum concentration to 20%, but I just cannot stop the cell dying. Are there any one ever working with this cell line and can give me some suggestions? Thank you!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.