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fluorescence labeling


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#1 Carpetto

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Posted 20 December 2010 - 07:33 AM

Hi everyone,

I would like to label the statins simvastatin and rosuvastatin to any fluorescent dye ( I can almost detect every dye). Please find attached both statins complexed to the HMG-CoA-Reductase (target of the statins).
For simvastatin, there is a COOH-group that may be a good link. However, a spacer may be necessary. Further information can be find here http://www.pdb.org/p...tructureId=1HW9
For rosuvastatin I guess the CH3-group could work. Further informations http://www.pdb.org/p...tructureId=1HWL
In both cases I do not know which dye to choose and how to link it to the statins. Unfortunately, I am not a chemist but studied biotechnology so I have some ideas but not expert ideas.
So could anybody help?

Thanks a lot!

PS: You can also answer in German.

Attached Thumbnails

  • HMGCR_Rosuvastatin_Complex.jpg
  • HMGCR_Simvastatin_Complex.jpg


#2 sgt4boston

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Posted 30 December 2010 - 03:34 AM

Pierce-Thermo Fisher makes cross-linking reagents and has a good tecnical staff to help with crosslinkers etc....

http://www.piercenet...CFQJN4Aod7Qming


http://www.piercenet...C6-BD3A4956A553

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#3 mdfenko

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Posted 30 December 2010 - 06:45 AM

you can also look at the molecular probes (invitrogen) handbook.
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