What is SOB containing 20 mM MgSO4 ?
Posted 20 December 2010 - 01:04 AM
I am preparing SOB media containing 20 mM MgSO4. I read several protocols that make me confuse.
Some recipe is MgCl2 (10 mL/L of 1M MgCl2) and MgSO4 (20 mL/L of 1M MgSO4).
Another is only MgSO4 (20 mL/L of 1M MgSO4).
But some is MgCl2 (10 mL/L of 1M MgCl2) and MgSO4 (10 mL/L of 1M MgSO4).
If the SOB media will be used for preparing competent cells, which recipe is the correct one.
I do appreciate your kind assistance in advance.
Thank you very much
Posted 20 December 2010 - 01:20 PM
Posted 21 December 2010 - 07:12 AM