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What is SOB containing 20 mM MgSO4 ?


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#1 RNasefree

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Posted 20 December 2010 - 01:04 AM

Hello,

I am preparing SOB media containing 20 mM MgSO4. I read several protocols that make me confuse.

Some recipe is MgCl2 (10 mL/L of 1M MgCl2) and MgSO4 (20 mL/L of 1M MgSO4).
Another is only MgSO4 (20 mL/L of 1M MgSO4).
But some is MgCl2 (10 mL/L of 1M MgCl2) and MgSO4 (10 mL/L of 1M MgSO4).

If the SOB media will be used for preparing competent cells, which recipe is the correct one.

I do appreciate your kind assistance in advance.
Thank you very much
Urai

#2 bob1

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Posted 20 December 2010 - 01:20 PM

SOB (super-optimal broth) with 20 mM Mg2+, composed of 10 mM MgCl2 and 10 mM MgSO4 is the correct formulation. Read Hanahan 1983, J. Mol Biol 166; 557-580 for more information.

#3 philman

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Posted 21 December 2010 - 07:12 AM

I think it is the total concentration of Mg2+ which is important, there are various different recepies using different salts to give the 20mM Mg2+ but they should all give similar results.




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