Hi!!
Anyone know if it is important the orientation of this gene? I want to do a fusion protein with this tag, and the transcription of my gene is from right to left, and I want to put the bio-tag in the N-terminal, so...I must put the biotag gene in the same orientation or it is no neccesary?
thank you!!!!
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mdfenko
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an elder
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Excellent
Excellent
Posted 20 December 2010 - 07:30 AM
yes, you must maintain the proper orientation and frame if you want to produce a fusion protein.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
