3 replies to this topic

[rimal]

hi all

I have RNA and purified protein can I do immunopricipitation to detect if my protein can bind to this RNA? or IP is designed only to detect complexes from lysates?  any suggestions are wellcome

[bob1]

Check out EMSA (electrophoretic mobility shift assay) protocols - designed for DNA, but they should be adaptable to RNA.

[rimal]

thanks for your reply, EMSA is very tricky, but can I do IP + RT PCR?

[knuf]

What about like, a Chromatin IP (ChIP)? I don't know much about the procedure, but it seems like this would be the time to do it.

Edited by kfunk106, 06 January 2011 - 01:33 PM.