Disruption of cells using mild detergent
Posted 18 December 2010 - 12:28 PM
I am looking for an effective way to disrupt mammalian white blood cells (physical shearing such as a beadmill is not possible at this stage) without actually purifying the DNA... As such i am considering a mild detergent such as 0.05-0.5% Tween 20 or Triton-X and perhaps some some mild vortexing.... Do you think this will be sufficient for this purpose? Another option might be simply several freeze/thaws however i don't know how efective this will be... Any ideas or suggestions?
Posted 18 December 2010 - 01:09 PM
For protein collection from HEK cells, I use a buffer with 0.5% NP-40 and do 3 freeze-thaw cycles with pretty good yield
Posted 18 December 2010 - 07:36 PM