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Eruption of mammalian cells using detergent


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#1 DavidA

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Posted 18 December 2010 - 12:23 PM

Hey guys,

I am looking for an effective way to disrupt mammalian white blood cells (physical shearing such as a beadmill is not possible at this stage) without actually purifying the DNA... As such i am considering a mild detergent such as 0.05-0.5% Tween 20 or Triton-X and perhaps some some mild vortexing.... Do you think this will be sufficient for this purpose? Another option might be simply several freeze/thaws however i don't know how efective this will be... Any ideas or suggestions?
Thanks

#2 lab rat

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Posted 18 December 2010 - 05:07 PM

One freeze-thaw in a lysis buffer will be more than sufficient. It depends on your end purpose what lysis buffer to use.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.




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