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Differential display versussubtraction hybridization


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#1 anonymous

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Posted 23 October 2001 - 09:00 PM

What is the best method to compare the gene expression of two lines of chicken?Differential display or subtraction hybridization?

Thanks!


#2 anonymous

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Posted 29 November 2001 - 10:00 PM

I think that depends on how much work you want to put into it. I personally think that the analysis of subtracted libraries is easier than cutting out all those bands out of poylacrylamide gels used in differential display. But, differential display has the advantage of having an amplifaction inherent in it since it is a PCR technique. Clontech makes a pretty cool kit that seems to combine the advantages of both. It is a subtraction procedure, but it has an amplification step. You should check it out- in fact I have the kit and am about ready to start an experiment with it. Here is the website:[url="http://www.clontech.com/products/catalog01/Sec1/p37cdnasubtract.html"][url]http://www.clontech.com/products/catalog01...nasubtract.html[/url][/url]

#3 anonymous

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Posted 29 November 2001 - 10:00 PM

I think that depends on how much work you want to put into it. I personally think that the analysis of subtracted libraries is easier than cutting out all those bands out of poylacrylamide gels used in differential display. But, differential display has the advantage of having an amplifaction inherent in it since it is a PCR technique. Clontech makes a pretty cool kit that seems to combine the advantages of both. It is a subtraction procedure, but it has an amplification step. You should check it out- in fact I have the kit and am about ready to start an experiment with it. Here is the website:[url="http://www.clontech.com/products/catalog01/Sec1/p37cdnasubtract.html"][url]http://www.clontech.com/products/catalog01...nasubtract.html[/url][/url]




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