Using atT20 cells to transfect GFP-plasmid, we can not get good results even we used different method for transfection (calcium precipitation, liposomes, detergent...). We will be grateful to have advices in this field.
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transfection of atT20 cells
1 reply to this topic
Posted 08 May 2002 - 12:07 PM
Check previously effective protocols. If every option gives poor results check your passage number. Perhaps it's time to start a fresh culture. Also, test for mycoplasma contamination. Many test kits are commercially available.