Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

transfection of atT20 cells


  • Please log in to reply
1 reply to this topic

#1 pasquet

pasquet

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 02 May 2002 - 09:58 PM

Using atT20 cells to transfect GFP-plasmid, we can not get good results even we used different method for transfection (calcium precipitation, liposomes, detergent...). We will be grateful to have advices in this field.
Thank you.

#2 milanoj

milanoj

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 72 posts
0
Neutral

Posted 08 May 2002 - 12:07 PM

Check previously effective protocols. If every option gives poor results check your passage number. Perhaps it's time to start a fresh culture. Also, test for mycoplasma contamination. Many test kits are commercially available.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.