I have to make Tris buffer with EDTA of pH 8.0. I am little confused with either to add the EDTA before adjusting the pH or first adjust the pH and then put the but the EDTA.
I shall be very thankful to you for your kind reply.
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lab rat
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Posted 16 December 2010 - 04:42 AM
You can adjust after adding the EDTA. If you are using Tris-base, the EDTA will lower the pH. You can make adjustments after adding the EDTA.
Edited by lab rat, 16 December 2010 - 04:45 AM.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.
