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16 replies to this topic

#16 ujla80

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Posted 20 December 2010 - 04:13 PM

sorry didnot understant..what is specs..VC?



It's possible to tell if the two samples are statistically different from each other if the the specs ( as VC at the same level) from the method are known.
We had a simular problem and after consulting our biostatistic department we could tell if the groups where different or not.
As I remember one of the big issues was the presence of very abnormal values in 1 one of the samples and the confidence of the test depended strongly of the number of samples in the pool.


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#17 bob1

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Posted 20 December 2010 - 06:12 PM

I think that Gerard was talking about the error of the machine you used to measure the assay on. This is still not really valid for statistical analysis as it doesn't prove anything - other than that you measured two samples and they are different... you can make no claims about that biologically, as it could have been that one of your pooled samples was really different from all the others - which is giving a flase impression of the differences.




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