Hi,
Could anyone explain why HEK393T grow much faster than HEK293? Besides transfection efficiency, is there any difference between this two cell lines?
I am a starter for mammalian cell biology. Many thanks for kind answers.
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#2
pcrman
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Posted 15 December 2010 - 09:37 PM
293T cells are subline of 293 cells and stably express SV40 large T antigen, G418 resistant.
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CCWW
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Posted 16 December 2010 - 10:20 AM
Thank you very much for your answer. But do you happen to know why the expression of largr T antigen can make the cell grow faster?
pcrman, on 15 December 2010 - 09:37 PM, said:
293T cells are subline of 293 cells and stably express SV40 large T antigen, G418 resistant.
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bob1
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Posted 16 December 2010 - 08:13 PM
T-Ag binds to Rb1 and several other proteins that inhibit cell cycle, such that the cell is forced into DNA synthesis/cycling again.
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Posted 17 December 2010 - 01:55 PM
Thank you. This makes sense.
bob1, on 16 December 2010 - 08:13 PM, said:
T-Ag binds to Rb1 and several other proteins that inhibit cell cycle, such that the cell is forced into DNA synthesis/cycling again.
