I am doing toxicity experiments,but I have a problem. I usually add my drug to the cells and icubate it for a certain time, then measure the absorbance and compare it with the control, which has no drug in it. But I can see that some of the cells of my control are dead as well, I can see some holes, which means some of the cells have come off. Do you know why this might be?
Death of cells in the control.
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