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total protein assay in cell extracts


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#1 hhly

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Posted 16 February 2002 - 03:23 AM

what is the best methods of total protein concentration  in mamal cell extracts

#2 flashboy

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Posted 19 February 2002 - 06:23 AM

i use the dc protein assay available from biorad.  it is very quick, easy and reliable.

#3 Sean Peel

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Posted 21 February 2002 - 07:18 AM

I have used the DC assay (based on the Lowry method) the Bradford assay (also available from BioRad or Pierce) and the BCA assay (Pierce). All in microplate format
My preference is the Bradford assay but the BCA works well also. I found the Lowry assay had the lowest sensitivity and I believe it has the largest protein to protein variation. One problem with the Bradford assay is that the filiter required for a multiwell plate reader (595nm) is not standard so you ma not have it already.




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